Chicken Leptin Receptor Protein

SKU: orb426842

Description

Recombinant of chicken Leptin Receptor protein

Images & Validation

• Application Notes:
  Protein content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard

Key Properties

• Source: Escherichia Coli
• Protein Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Asp-Val-Asn-Ile Biological ActivityBiological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio
• Purity: Greater than 99.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.

Storage & Handling

• Storage: Stability: Sterile solutions at 0.5mg/ml or less are stable at 4°C for several months
• Form/Appearance: Sterile Filtered colorless solution at a concentration of 0.4 mg/ml.
• Buffer/Preservatives: The protein was filter sterilized and stored at 4°C (0.2 to 0.5 mg/ml) solution of Tris-HCl buffer, pH 9.0 with 150mM NaCl.
• Disclaimer: For research use only

Alternative Names

OB Protein, Obesity Protein, OBS, Obesity factor, Leptin Receptor.