Chicken IgG F(ab')2

SKU: orb2652772

Description

Chicken IgG F(ab')2

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Chicken IgG F(ab')2 fragment has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments

Key Properties

• Source: Chicken
• Biological Origin: Chicken
• Isotype: IgG F(ab')2
• Conjugation: Unconjugated
• Purity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG, anti-Chicken IgG F(ab’)2 and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(c) or anti-Pepsin.

Storage & Handling

• Storage: Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Chicken IgG F(ab')2 fragment, Chicken IgG Fab2 fragment, Chicken IgY F(ab')2 fragment, Chicken IgY Fab2 fragment

SDS-PAGE of Chicken IgG/IgY Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Reduced Chicken IgG Fab Fragment (p/n orb2652771). Lane 4: Reduced Chicken IgM Whole Molecule (p/n orb346054). Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.

SDS-PAGE Results of Chicken IgG F(ab)'2. Lane 1: Chicken IgG F(ab)'2 fragment - Reduced [5 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Chicken IgG F(ab)'2 fragment - Non-Reduced [5 µg]. 4-20% Gel. Coomassie stained.