CHI3L2 Rabbit Polyclonal Antibody

• Catalog Number: orb158727
• Category: Antibodies
• Description: CHI3L2 Rabbit Polyclonal Antibody
• Target: CHI3L2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human YKL39/CHI3L2 (121-220/390aa)
• UniProt ID: Q15782
• MW: 41 kDa
• Tested applications: ELISA, ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CH3L2_HUMAN; CHI3L2; Chitinase 3 like 2; Chitinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CHI3L2) Polyclonal Antibody, Unconjugated (orb158727) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.