CHI3L1 Rabbit Polyclonal Antibody

• Catalog Number: orb182759
• Category: Antibodies
• Description: CHI3L1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CHI3L1 (301-383/383aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: CHI3L1
• UniProt ID: P36222
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CHI3L1; 39 kDa synovial protein; ASRT7; Cartilage
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Cell: HepG2, Concentration: 1:100, Host/Isotype: Rabbit/IgG, Flow cytometric analysis of Rabbit IgG isotype control (Cat#: orb182759) on HepG2 (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

Sample: HepG2 (Human) Cell Lysate at 30 ug Recombinant human CHI3L1 Protein at 2 ug Primary: Anti-CHI3L1 (orb182759) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD.

Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-CHI3L1 (orb182759) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.