CHI3L1 Rabbit Polyclonal Antibody

• Catalog Number: orb10365
• Category: Antibodies
• Description: CHI3L1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, WB
• Predicted Reactivity: Human, Mouse
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from rat CHI3L1 (330-381/381aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ICC/IF=1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: CHI3L1
• UniProt ID: Q9WTV1
• RRID: AB_10751372
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CHI3L1; 39 kDa synovial protein; ASRT7; Cartilage
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Protein: Liver (Rat) lysate at 30 ug, Colon carcinoma (Human) lysates at 30 ug, Primary: Anti-CGP-39/CHI3L1/YKL-40 (orb10365) at 1:200, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000, Predicted band size: 42kD, Observed band size: 39kD.

Sample: Lane 1: Mouse Lung tissue lysates, Lane 2: Rat Lung tissue lysates, Primary: Anti-CHI3L1 (orb10365) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kDa, Observed band size: 42 kDa.

Tissue/Cell: human endometrium carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CGP-39/ CHI3L1/YKL-40 Polyclonal Antibody, Unconjugated (orb10365) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CGP-39/ CHI3L1/YKL-40 Polyclonal Antibody, Unconjugated (orb10365) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

U87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (orb10365) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.