CHFR antibody

• Catalog Number: orb18717
• Category: Antibodies
• Description: Goat polyclonal antibody to CHFR
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:64000, WB: 0.5ug/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 73.4; 73.3; 72.0; 69.2; 63.9
• Target: CHFR
• Entrez: 55743
• Protein Sequence: KFNHICEQTRFKN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CHFR antibody, anti checkpoint with forkhead
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded human brain (cerebellum) shows stained Purkinje cells. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5 μg/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human brain using CHFR antibody