CHCHD3 Antibody

• Catalog Number: orb1249171
• Category: Antibodies
• Description: CHCHD3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat, Sheep
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RSSEFYRVTTEQYQ
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CHCHD3
• UniProt ID: Q9NX63
• NCBI: NP_060282.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CHCHD3, coiled-coil-helix-coiled-coil-helix domain
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26.2kDa band observed in Human Placenta lysates (calculated MW of 26kDa according to NP_060282.1). Recommended concentration: 0.3-1ug/ml. A customer reported ~30kDa in THP1 and in human primary monocyte derived dendritic cells.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

orb1249171 (0.3 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249171 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.