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Catalog Number | orb2397702 |
---|---|
Category | Antibodies |
Description | CHAC1 Rabbit Polyclonal Antibody (BF488) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF |
Predicted Reactivity | Bovine, Canine, Human, Porcine, Rabbit |
Reactivity | Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human CHAC1 (131-264/264aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IF=1:100-500 |
Conjugation | BF488 |
Target | CHAC1 |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Cation transport regulator like protein 1; Cation Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-CHAC1 antibody (orb2397702), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.