CH25H Rabbit Polyclonal Antibody

• Catalog Number: orb1152140
• Category: Antibodies
• Description: CH25H Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CH25H (1-100/272aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 32 kDa
• Target: CH25H
• UniProt ID: O95992
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: C25H; Cholesterol 25 hydroxylase; Cholesterol 25-h
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Human U-87 MG cell Lysates, Primary: Anti-CH25H (orb1152140) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32kDa, Observed band size: 32kDa.