CGRP-I Rabbit Polyclonal Antibody

• Catalog Number: orb182870
• Category: Antibodies
• Description: CGRP-I Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Gallus, Human, Porcine
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CGRP-I (83-119/128aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 3.5 kDa
• Target: CALCA
• UniProt ID: P01258
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CALCA_HUMAN; Calcitonin gene-related peptide 1; al
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Duarte, Felipe C. K. et al. Experimentally induced spine osteoarthritis in rats leads to neurogenic inflammation within neurosegmentally linked myotomes Exp Gerontol, 149, 111311 (2021)

PubMed: 33744392

Applications

WB

Reactivity

Rat

Ahmadi, Bahareh et al. Ultrasonographic assessment of skeletal muscles after experimentally induced neurogenic inflammation (facet injury) in rats Exp Biol Med (Maywood), (2022)

PubMed: 36113006

Applications

WB

Reactivity

Rat

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CGRP-I) Polyclonal Antibody, Unconjugated (orb182870) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CGRP-I) Polyclonal Antibody, Unconjugated (orb182870) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CGRP-1 Polyclonal Antibody, Unconjugated (orb182870) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.