CGKI Rabbit Polyclonal Antibody

• Catalog Number: orb101133
• Category: Antibodies
• Description: CGKI Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Prkg1/cGKI (551-650/671aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ICC/IF=1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 74 kDa
• Target: PRKG1
• UniProt ID: Q13976
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: cGKI alpha; CGKI; cGKI beta; cGMP-dependent protei
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (cGKI) polyclonal Antibody, Unconjugated (orb101133) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Heart (Mouse) Lysate at 40 ug, Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-cGKI (orb101133) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74 kD, Observed band size: 74 kD.

Sample: Hela Cell Lysate at 40 ug, U2OS Cell Lysate at 40 ug, Primary: Anti-cGKI (orb101133) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74 kD, Observed band size: 74 kD.