CGA Antibody

• Catalog Number: orb1252409
• Category: Antibodies
• Description: CGA Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HCGab/52
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Purified hCG protein was used as the immunogen for the hCG Holo antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the hCG Holo antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CGA
• UniProt ID: P01215, P01233
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Glycoprotein hormones alpha chain, Anterior pituit
• Note: For research use only
• Application notes: Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the hCG Holo antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human placenta stained with hCG Holo antibody (HCGab/52).

SDS-PAGE Analysis of Purified, BSA-Free hCG Holo Antibody (clone HCGab/52). Confirmation of Integrity and Purity of the Antibody.