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Catalog Number | orb1252124 |
---|---|
Category | Antibodies |
Description | CG alpha Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CGA91-2 |
Tested applications | IHC |
Reactivity | Human |
Isotype | IgG1, kappa |
Immunogen | Recombinant protein was used as the immunogen for this HCG-alpha antibody. |
Concentration | 0.2 mg/mL |
Dilution range | IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Variations in protocols, secondaries and substrates may require the HCG-alpha antibody to be titered for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CG alpha |
UniProt ID | P01215 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Glycoprotein hormones alpha chain, Anterior pituit Read more... |
Note | For research use only |
Application notes | IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Variations in protocols, secondaries and substrates may require the HCG-alpha antibody to be titered for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of human placenta stained with HCG-alpha antibody (CGA91-2).
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