CFTR Antibody

• Catalog Number: orb1274272
• Category: Antibodies
• Description: CFTR Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CFTR/1775R
• Tested applications: IF, IHC-P
• Reactivity: Human
• Isotype: IgG, k
• Immunogen: A recombinant partial protein corresponding to amino acids 258-385 from the human protein was used as the immunogen for this recombinant CFTR antibody.
• Concentration: 0.2 mg/mL
• Dilution range: IF: 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CFTR antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CFTR
• UniProt ID: P13569
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CFTR Antibody: CF, MRP7, ABC35, ABCC7, CFTR/MRP, T
• Note: For research use only
• Application notes: IF: 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CFTR antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas with recombinant CFTR antibody (clone CFTR/1775R). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.