CFLAR Antibody

• Catalog Number: orb1239385
• Category: Antibodies
• Description: CFLAR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: FLIP antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human FLIP.The sequence is identical in all FLIP splice variants.The immunogen is located within the first 50 amino acids of FLIP.
• Concentration: 1 mg/mL
• Dilution range: FLIP antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 53 kDa Observed: 53 kDa
• Target: CFLAR
• UniProt ID: O15519
• NCBI: AAC51622
• Storage: FLIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Cas
• Note: For research use only
• Application notes: FLIP antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FLIP in (A) HeLa cell and (B) mouse liver tissue lysate with FLIP antibody at 1 µg/ml.

Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/mL.

Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 µg/mL.

Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 µg/mL.

Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20 µg/ml. Red: FLIP Antibody (orb1239385) Blue: DAPI staining.