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Catalog Number | orb341421 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CFL2 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant full length protein of human Cofilin 2 |
Dilution range | WB: 1:500-1:2000, IF/ICC: 1:50-1:100 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | CFL2 |
Entrez | 12632, 1073 |
UniProt ID | Q9Y281, P45591 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | Cofilin-2; Cofilin, muscle isoform Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Cofilin 2 expression in Hela (A), mouse heart (B), rat kidney (C), rat brain (D) whole cell lysates. (Predicted band size: 16; 18 kD; Observed band size: 18 kD)
Immunofluorescent analysis of Cofilin 2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Mouse, Porcine | |
Human, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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