CFL2 Antibody

• Catalog Number: orb1247995
• Category: Antibodies
• Description: CFL2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse, Porcine
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: C-DIKDRSTLGEK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 18kDa band observed in Human Skeletal Muscle lysates and in Rat Heart lysates (calculated MW of 18.7kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CFL2
• UniProt ID: Q9Y281
• NCBI: NP_068733.1, NP_619579.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: cofilin 2 (muscle) , HGNC:1875, CFL2, NEM7, cofili
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 18kDa band observed in Human Skeletal Muscle lysates and in Rat Heart lysates (calculated MW of 18.7kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247995 (0.003 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Human Muscle Cofilin 2 and probed with orb1247995 (mock transfection in first lane).

orb1247995 (0.001 ug/ml) staining of Rat Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247995 (3.75 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.