CF150 Antibody

• Catalog Number: orb1270774
• Category: Antibodies
• Description: CF150 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This CF150 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human CF150.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 59 kDa
• Target: MB21D1
• UniProt ID: Q8N884
• NCBI: Q8N884
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Cyclic GMP-AMP synthase, cGAMP synthase, cGAS, h-c
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For WB starting dilution is: 1:500-1:1000
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (NA168821) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1 ug/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Flow cytometric analysis of A549 cells using CF150 Antibody (green) compared to an isotype control of rabbit IgG (blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysate from THP-1 cell line, using CF150 Antibody at 1:1000.

Western blot analysis of lysates from MCF-7, HT-29, THP-1 cell line, human spleen, human cerebellum tissue lysate (from left to right), using CF150 Antibody at 1:1000 at each lane.

Western blot analysis of lysates from HT-29, THP-1 cell line, human cerebellum tissue lysate (from left to right), using CF150 Antibody at 1:1000 at each lane.

Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.