CES4A Rabbit Polyclonal Antibody

SKU: orb182560

Description

CES4A Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CES4A (491-561/561aa)
• Target: CES4A
• Molecular Weight: 61 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Carboxylesterase 4A; Carboxylesterase 6; Carboxylesterase 8(putative); Ces4a; CES6; CES8; EC 3.1.1.-; EST4A_HUMAN; FLJ37002; FLJ37464; OTTHUMP00000237118; OTTHUMP00000237120; OTTHUMP00000237122; OTTHUMP00000237123.

Sample: Brain (Mouse) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-CES4A (orb182560) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kD, Observed band size: 50 kD.

Tissue/Cell: human cervical cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CES4A Polyclonal Antibody, Unconjugated (orb182560) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CES4A

UniProt

Q5XG92