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Ceramide glucosyltransferase Rabbit Polyclonal Antibody

Catalog Number: orb10701

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10701
CategoryAntibodies
DescriptionCeramide glucosyltransferase Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Rat
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human UGCG (21-120/394aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW44 kDa
TargetUGCG
UniProt IDQ16739
RRIDAB_10750807
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCEGT_HUMAN; UGCG; GCS; GLCT 1; GLCT-1; GLCT1; Gluc
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Background_CLIENT_OVERRIDE_
NoteFor research use only
Expiration Date12 months from date of receipt.
Ceramide glucosyltransferase Rabbit Polyclonal Antibody

Blank control: mouse splenocytes (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Ceramide glucosyltransferase Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (orb10701) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Ceramide glucosyltransferase Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (orb10701) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Ceramide glucosyltransferase Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (orb10701) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Ceramide glucosyltransferase Rabbit Polyclonal Antibody

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GCS/glucosylceramide synthase Polyclonal Antibody, Unconjugated (orb10701) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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