CEP55 (phospho Ser425) rabbit pAb

• Catalog Number: orb769587
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CEP55 (phospho-Ser425)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: Synthesized phospho-peptide around the phosphorylation site of human CEP55 (phospho Ser425)
• MW: 54kD
• Tested applications: ELISA, IHC-P, WB
• Dilution range: WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CEP55 antibody, anti-C10orf3 antibody, anti-U
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Western Blot analysis of 1 HEK-293 cell, 2, LPS 100ng/mL 30min treated , using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000.