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Catalog Number | orb10371 |
---|---|
Category | Antibodies |
Description | CEBP Beta Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Bovine, Gallus, Porcine, Rabbit, Rat, Sheep |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human CEBP Beta (251-345/345aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 36 kDa |
Target | CEBPB |
UniProt ID | P17676 |
RRID | AB_10752832 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | CEBP-beta; CLEBP beta; CCAAT Enhancer Binding Prot Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control (blue line): Hela, Primary Antibody (green line): Rabbit Anti-CEBP Beta antibody (orb10371), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CEBP Beta) Polyclonal Antibody, Unconjugated (orb10371) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: HepG2 (Human) Cell Lysate at 40 ug, Primary: Anti-CEBP Beta (orb10371) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD.
Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-CEBP Beta (orb10371) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD.
IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC | |
Bovine, Canine, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Canine, Human, Porcine, Rabbit | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC-Fr, IHC-P | |
Human | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-Fr, IHC-P, WB | |
Bovine, Gallus, Porcine, Rabbit, Rat, Sheep | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
HRP |