CEA Antibody [SPM584]

• Catalog Number: orb1251949
• Category: Antibodies
• Description: CEA Antibody [SPM584]
• Target: CEACAM5
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: Human colon carcinoma extract was used as the immunogen for this CEA antibody.
• UniProt ID: P06731
• Tested applications: FC, IF, IHC, WB
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the CEA antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: SPM584
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CEACAM5 Antibody : CEA, CD66e, CEA, Carcinoembryon
• Research Area: Cancer Biology, Cell Biology
• Note: For research use only

Formalin-fixed, paraffin-embedded human colon carcinoma stained with CEA antibody (SPM584).

Western blot of human human stomach lysate using CEA antibody (SPM584).