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    CDKN2A antibody

    Catalog Number: orb129709

    DispatchUsually dispatched within 5-10 working days.
    $ 507.00
    Catalog Numberorb129709
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody against CDKN2A
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, IF, WB
    ReactivityHuman
    IsotypeIgG
    ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-156 of human CDKN2A/p16INK4a (NP_000068.1).
    Concentration1 mg/ml
    Dilution rangeWB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
    ConjugationUnconjugated
    MW16kDa
    UniProt IDQ8N726, P42771
    StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
    Buffer/PreservativesPBS with 0.05% proclin300,50% glycerol,pH7.3.
    Alternative namesanti-ARF antibody, anti-CCM2 antibody, anti-CDK4 i
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    CDKN2A antibody

    Immunofluorescence analysis of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit pAb (orb129709) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

    CDKN2A antibody

    Western blot analysis of lysates from HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit pAb (orb129709) at 1:18000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

    CDKN2A antibody

    Western blot analysis of lysates from HeLa cells, using [KO Validated] CDKN2A/p16INK4a Rabbit pAb (orb129709) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

    CDKN2A antibody

    Western blot analysis of lysates from wild type(WT) and CDKN2A/p16INK4a knockout (KO) 293T(KO) cells, using [KO Validated] CDKN2A/p16INK4a Rabbit pAb (orb129709) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

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