CDKN2A Antibody

• Catalog Number: orb1261314
• Category: Antibodies
• Description: CDKN2A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human CDKN2A / p16INK4a (NP_000068.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 18kDa
• Target: CDKN2A
• UniProt ID: Q8N726, P42771
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CDKN2A Antibody: ARF, MLM, P14, P16, P19, CMM2, IN
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of 293T cells, using CDKN2A / p16INK4a antibody (orb1261314). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug CDKN2A/p16INK4a antibody (orb1261314). Western blot was performed from the immunoprecipitate using CDKN2A/p16INK4a antibody (orb1261314) at a dilution of 1:1000.