CDKN1B Antibody

• Catalog Number: orb395397
• Category: Antibodies
• Description: Rabbit polyclonal antibody to KIP1.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: cyclin-dependent kinase inhibitor 1B(p27, Kip1)
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:200-1:2000; IP: 1:200-1:1000; IHC: 1:50-1:500; IF: 1:50-1:500
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: CDKN1B
• UniProt ID: P46527
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27, KIP1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with orb395397 (P27; KIP1 Antibody) at dilution of 1:600.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using orb395397 (P27; KIP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-P27; KIP1 (IP: orb395397, 4 ug; Detection: orb395397 1:300) with NIH/3T3 cells lysate 1600 ug.

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb395397 (P27; KIP1 Antibody) at dilution of 1:200. heat mediated antigen retrieved with Tris-EDTA buffer (pH9).