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Catalog Number | orb1259414 |
---|---|
Category | Antibodies |
Description | CDKN1B Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p27 KIP 1 (NP_004055.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 30kDa |
Target | CDKN1B |
UniProt ID | P46527 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Cyclin-dependent kinase inhibitor 1B, Cyclin-depen Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using p27 KIP 1 antibody (orb1259414) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using p27 KIP 1 antibody (orb1259414) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using p27 KIP 1 antibody (orb1259414) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using p27 KIP 1 antibody (orb1259414) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using p27 KIP 1 antibody (orb1259414) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using p27 KIP 1 antibody (orb1259414) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using p27 KIP 1 antibody (orb1259414) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using p27 KIP 1 antibody (orb1259414) at dilution of 1:100 (40x lens).
FC, IF, IHC-P, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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