CDKN1B Antibody

• Catalog Number: orb1252597
• Category: Antibodies
• Description: CDKN1B Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KIP1/769
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the p27Kip1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the p27Kip1 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CDKN1B
• UniProt ID: P46527
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Cyclin-dependent kinase inhibitor 1B, Cyclin-depen
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the p27Kip1 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with p27Kip1 antibody (KIP1/769).

Formalin-fixed, paraffin-embedded human prostate cancer stained with p27Kip1 antibody (KIP1/769).

Formalin-fixed, paraffin-embedded human cervical cancer stained with p27Kip1 antibody (KIP1/769).

Flow cytometry testing of Jurkat cells. Black: cells alone; Grey: isotype control; Green: Alexa Fluor 488-labeled p27Kip1 antibody (KIP1/769).

Formalin-fixed, paraffin-embedded rat colon stained with p27Kip1 antibody (KIP1/769).