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CDKN1A/P21 Rabbit Polyclonal Antibody (BF647)
Description
Images & Validation
−| Tested Applications | FC, ICC, IF |
|---|---|
| Dilution range | Flow-Cyt=1μg/Test, IF=1:100-500, ICC/IF=1:100-500 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Bovine, Canine, Gallus, Mouse |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human P21 (21-100/164aa) |
| Target | CDKN1A |
| Purification | Affinity purified by Protein A |
| Conjugation | BF647 |
Storage & Handling
−| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Tissue/Cell: HUVEC cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal rabbit serum) at 37°C for 20 min, Incubation: Anti-CDKN1A/P21 Polyclonal Antibody, conjugated (orb1583071) 1:50, 2 hours at 37°C, DAPI (blue) was used to stain the cell nuclei.
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CDKN1A/P21 Rabbit Polyclonal Antibody (BF647) (orb1583071)
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