CDK4 Rabbit Polyclonal Antibody (FITC)

SKU: orb15344

Description

CDK4 Rabbit Polyclonal Antibody (FITC) is an FITC conjugated antibody targeting CDK4. This antibody is suitable for FC, ICC, IF. It exhibits reactivity with Human, Mouse, Rat samples.

Research Area

Cell Biology, Cyclin-dependent kinase 4 (CDK4), Epigenetics and Nuclear Signaling

Images & Validation

• Tested Applications: FC, ICC, IF
• Dilution range: Flow-Cyt=1μg/Test, IF=1:100-500, ICC/IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CDK4 (241-303/303aa)
• Target: CDK4
• Purification: Affinity purified by Protein A
• Conjugation: FITC

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Cdk 4; CDK4 protein; Cell division kinase 4; Cell division protein kinase 4; CMM 3; CMM3; Crk3; Cyclin dependent kinase 4; Melanoma cutaneous malignant 3; MGC14458; p34 cdk4; PSK J3; CDK4_HUMAN.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-CDK4 antibody (orb15344), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

CDK4