CDK4 Antibody

• Catalog Number: orb2636240
• Category: Antibodies
• Description: Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level (PMID:17253961). CCND1- CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth (PMID:21844184). CDK4 is highly expressed in highly differentiated and dedifferentiated liposarcomas, but rarely expressed in other benign liposarcomas and other sarcomas. CDK4 and MDM2 combined to differentiate between highly differentiated liposarcoma (+), dedifferentiated liposarcoma (+) and myxoid liposarcoma, pleomorphic liposarcoma, spindle lipoma, pleomorphic lipoma and other high-grade sarcomas.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant fragment (within amino acids 150-303) of human Cyclin-dependent kinase 4 protein was used as the immunogen for the Cyclin-dependent kinase 4 antibody.
• UniProt ID: P11802
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the Cyclin-dependent kinase 4 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CDK4/8896
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Cyclin-dependent kinase 4 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human colon carcinoma tissue with Cyclin-dependent kinase 4 antibody (clone CDK4/8896). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Cyclin-dependent kinase 4 antibody (clone CDK4/8896) as confirmation of integrity and purity.