CDH16 Antibody

• Catalog Number: orb1274233
• Category: Antibodies
• Description: CDH16 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CDH16/1532R
• Tested applications: FC, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: IgG, kappa
• Immunogen: Human protein was used as the immunogen for the recombinant Cadherin 16 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10e6 cellsImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Cadherin 16 antibody to be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CDH16
• UniProt ID: O75309
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Cadherin-16, Kidney-specific cadherin, Ksp-cadheri
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10e6 cellsImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Cadherin 16 antibody to be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human renal cell carcinoma with recombinant Cadherin 16 antibody (clone CDH16/1532R). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

IHC testing of FFPE mouse kidney tissue with recombinant Cadherin 16 antibody (clone CDH16/1532R). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

IHC testing of FFPE rat kidney tissue with recombinant Cadherin 16 antibody (clone CDH16/1532R). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.