CDH1 Antibody

• Catalog Number: orb1252364
• Category: Antibodies
• Description: CDH1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CDH1/1525
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Optimal dilution of the E-Cadherin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CDH1
• UniProt ID: P12830
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Cadherin-1, CAM 120/80, Epithelial cadherin, E-cad
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Optimal dilution of the E-Cadherin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human skin stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human placenta stained with E-Cadherin antibody (CDH1/1525).

Western blot analysis A) partial recombinant protein B) human stomach lysate using E-Cadherin antibody (CDH1/1525). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).