E Cadherin 1 CDH1 Antibody (monoclonal, 9G2)

• Catalog Number: orb547022
• Category: Antibodies
• Description: E Cadherin 1 CDH1 Antibody (monoclonal, 9G2)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9G2
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 130 kDa
• UniProt ID: P12830
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cadherin-1; CAM 120/80; Epithelial cadherin; E-cad
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-CA2 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

IF analysis of COL4A1/E Cadherin using anti-COL4A1/E Cadherin antibody.COL4A1/E Cadherin was detected in paraffin-embedded section of human colon cancer tissues.

IF analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in immunocytochemical section of A431 cells.

IF analysis of E Cadherin using anti-E Cadherin antibody. E-cadherin was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in paraffin-embedded section of human oesophagus squama cancer tissue.

IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in paraffin-embedded section of human oesophagus squama cancer tissue.

IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in paraffin-embedded section of human colon cancer tissue.

WB analysis of E Cadherin using anti-E Cadherin antibody.Lane 1:human placenta tissue; 2:human A549 cell; 3:human HEK293 cell.