Cdc7 Rabbit Polyclonal Antibody

SKU: orb4864

Description

Cdc7 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Epigenetics and Nuclear Signaling, Phosphatases

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Cdc7 (151-250/574aa)
• Target: CDC7
• Molecular Weight: 64 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Cdc 7; Cdc-7; CDC7 Kinase; CDC7 cell division cycle 7; CDC7 cell division cycle 7 like 1; CDC7 L1; Cdc7 like 1; CDC7 related kinase; CDC7L 1; CDC7L1; Cell division cycle 7 homolog; Cell division cycle 7 kinase; Cell division cycle 7 like protein 1; Cell division cycle 7 related protein kinase; HsCDC 7; HsCDC7; Hsk 1; Hsk1; HuCDC 7; CDC7_HUMAN.

Blank control (blue): 293T (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Cdc7/FITC Conjugated antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.

Tissue/Cell: human glioma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cdc7 Polyclonal Antibody, Unconjugated (orb4864) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CDC7

UniProt

O00311