CDC37 Antibody

SKU: orb67522

Description

Rabbit Anti-Human CDC37 Polyclonal

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution range: WB (1:2000), ICC/IF (1:200)
• Reactivity: Human
• Application Notes:
  A 1:2000 dilution of SPC-142 was sufficient for detection of cdc37 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Native human Cdc37, full length
• Target: CDC37
• Molecular Weight: 44.5kDa
• Purification: Protein A Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1.68 mg/ml
• Disclaimer: For research use only

Alternative Names

CDC37, Hsp90 co-chaperone Cdc37, Cell division control protein 37, Hsp90 chaperone protein kinase-targeting subunit, CDC37_HUMAN, CDC37A, p50, p50Cdc37, S. cerevisiae hypothetical protein CDC37

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:200 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of CDC37 protein using Rabbit Anti-CDC37 Polyclonal Antibody. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:2000.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

Quick Database Links

Gene Symbol

CDC37

UniProt

Q16543

NCBI Gene ID

11140

RefSeq (Protein):NP_008996.1