CDC37 Antibody

• Catalog Number: orb1262433
• Category: Antibodies
• Description: CDC37 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Rabbit Ig
• Immunogen: This CDC37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the Central region of human CDC37.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 44 kDa
• Target: CDC37
• UniProt ID: Q16543
• NCBI: Q16543
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Hsp90 co-chaperone Cdc37, Hsp90 chaperone protein
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: C6 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lane 5: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of CDC37 Antibody in MCF-7, A2058 cell line lysates (35 ug/lane)

CDC37 Antibody IHC analysis in formalin fixed and paraffin embedded skin followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.