Cdc25B Rabbit Polyclonal Antibody

• Catalog Number: orb4857
• Category: Antibodies
• Description: Cdc25B Rabbit Polyclonal Antibody
• Target: CDC25B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human Cdc25B (501-580/580aa)
• UniProt ID: P30305
• RRID: AB_10949032
• MW: 65 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cdc 25B; Cdc25b; Cdc-25b; CDC25HU2; Cdc25m2; Cell
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cdc25B) Polyclonal Antibody, Unconjugated (orb4857) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Mouse NIH/3T3 cell lysates, Lane 3: Mouse Sp2/0 cell lysates, Lane 4: Human A549 cell lysates, Lane 5: Human Daudi cell lysates, Lane 6: Human K562 cell lysates, Primary: Anti-Cdc25B (orb4857) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kDa, Observed band size: 60 kDa.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-Cdc25B (orb4857) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.