CD99L2 Polyclonal Antibody

• Catalog Number: orb1420367
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD99L2.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Conjugation: Unconjugated
• UniProt ID: Q8TCZ2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CD99 antigen-like protein 2, MIC2-like protein 1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: MCF-7 (Human) Cell Lysate at 30 ug. Primary: Anti- CD99L2 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 25 kD. Observed band size: 28 kD.

Tissue/cell: human gastric cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CD99L2 Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.