CD99 antibody

• Catalog Number: orb154388
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD99 which belongs to the CD99 family. It plays role in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. CD99 is expressed in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation.
• Clonality: Monoclonal
• Clone Number: 3B2/TA8
• Tested applications: CyTOF, FC
• Reactivity: Human
• Isotype: Mouse IgG2a kappa
• Immunogen: Human thymocytes
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD99
• Entrez: 4267
• UniProt ID: P14209
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-MIC2 antibody, anti-HBA71 antibody, anti-bloo
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Mass cytometry (surface staining) of human peripheral blood cells (after separation using Ficoll-Paque density gradient centrifugation) with anti-human CD99 (3B2/TA8) Eu153. Gated on singlets.

Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD99 (3B2/TA8) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD99 (3B2/TA8) purified antibody (concentration in sample 2 µg/ml) GAM APC.