CD99 Antibody

• Catalog Number: orb1252490
• Category: Antibodies
• Description: CD99 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HO36-1.1
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: IgM, kappa
• Immunogen: Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/mlFlow Cytometry: 5-10ul/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the CD99 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD99
• UniProt ID: P14209
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface ant
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlFlow Cytometry: 5-10ul/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the CD99 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human testicular carcinoma stained with CD99 antibody (HO36-1.1).

Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with CD99 antibody (HO36-1.1).

Western blot testing of Jurkat cell lysate with CD99 antibody (clone HO36-1.1). Expected molecular weight: ~20/32kDa (unmodified/glycosylated).