CD99 Antibody

• Catalog Number: orb1274236
• Category: Antibodies
• Description: CD99 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MIC2/1495R
• Tested applications: FC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG, kappa
• Immunogen: Human protein was used as the immunogen for the recombinant CD99 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 2-4 ug/million cells in 0.1mlImmunofluorescence: 2-4 ug/mlImmunohistochemistry (FFPE): 5-10 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Titering of the recombinant CD99 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD99
• UniProt ID: P14209
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface ant
• Note: For research use only
• Application notes: Flow Cytometry: 2-4 ug/million cells in 0.1mlImmunofluorescence: 2-4 ug/mlImmunohistochemistry (FFPE): 5-10 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Titering of the recombinant CD99 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human Ewing's sarcoma with recombinant CD99 antibody (clone MIC2/1495R). Required HIER: steam sections in 10mM Citrate buffer, pH 6.0, for 10-20 min.