CD99 Antibody

• Catalog Number: orb1252033
• Category: Antibodies
• Description: CD99 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CDLA99
• Tested applications: FC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the CD99 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the CD99 antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. Not suitable for rat samples testing.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD99
• UniProt ID: P14209
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface ant
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the CD99 antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. Not suitable for rat samples testing.
• Expiration Date: 12 months from date of receipt.

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