CD95/FAS Rabbit Polyclonal Antibody

• Catalog Number: orb10642
• Category: Antibodies
• Description: CD95/FAS Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from rat FAS (35-110/327aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=2μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 40-50 kDa
• Target: FAS
• UniProt ID: Q63199
• RRID: AB_10767244
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ALPS 1A; ALPS1A; APO 1; Apo-1; Apo 1 antigen; APO
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): Mouse Kidney (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-CD95/FAS antibody (orb10642, Green), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Blank control: Raji. Primary Antibody (green line): Rabbit Anti-CD95/FAS antibody (orb10642), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Thymus (Mouse) Lysate at 40 ug, Lane 2: Thymus (Rat) Lysate at 40 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: Raji (Human) Cell Lysate at 30 ug, Lane 5: HT1080 (Human) Cell Lysate at 30 ug, Lane 6: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-CD95/FAS (orb10642) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45/52 kD, Observed band size: 52 kD.

Sample: serum (Rat) at 40 ug, plasma (Rat) at 40 ug. Primary: Anti-CD95 (orb10642) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 34 kD, Observed band size: 42 kD.