CD95/FAS Rabbit Polyclonal Antibody

SKU: orb10642

Description

CD95/FAS Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from rat FAS (35-110/327aa)
• Target: FAS
• Molecular Weight: 40-50 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ALPS 1A; ALPS1A; APO 1; Apo-1; Apo 1 antigen; APO 1 cell surface antigen; Apo-1 antigen; APO1; Apo1 antigen; APO1 cell surface antigen; Apoptosis antigen 1; Apoptosis mediating surface antigen FAS; Apoptosis-mediating surface antigen FAS; APT 1; APT1; CD 95; CD 95 antigen; CD95 antigen; delta Fas; delta Fas/APO 1/CD95; delta Fas/APO1/CD95; FAS 1; FAS 827dupA; Fas AMA; FAS Antigen; FAS1; FASLG receptor; FASTM; TNF receptor superfamily, member 6; TNFRSF 6; TNFRSF6; TNR6_HUMAN; Tumor necrosis factor receptor superfamily member 6.

Blank control (blue): Mouse Kidney (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-CD95/FAS antibody (orb10642, Green), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Blank control: Raji. Primary Antibody (green line): Rabbit Anti-CD95/FAS antibody (orb10642), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Thymus (Mouse) Lysate at 40 ug, Lane 2: Thymus (Rat) Lysate at 40 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: Raji (Human) Cell Lysate at 30 ug, Lane 5: HT1080 (Human) Cell Lysate at 30 ug, Lane 6: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-CD95/FAS (orb10642) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45/52 kD, Observed band size: 52 kD.

Sample: serum (Rat) at 40 ug, plasma (Rat) at 40 ug. Primary: Anti-CD95 (orb10642) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 34 kD, Observed band size: 42 kD.

Quick Database Links

Gene Symbol

FAS

UniProt

Q63199