CD90/Thy1 Antibody

• Catalog Number: orb19300
• Category: Antibodies
• Description: CD90/Thy1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat Immunofluorescence, 5 μg/ml, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 18080 MW
• UniProt ID: P01831
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Thy-1 membrane glycoprotein;Thy-1 antigen;CD90;Thy
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HEPA1-6 cells using anti-CD90/Thy1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of CD90/Thy1 using anti-CD90/Thy1 antibody.Lane 1:mouse thymus tissue; 2:mouse brain tissue; 3:rat thymus tissue; 4:rat brain tissue.

IF analysis of CD90/Thy1 using anti-CD90/Thy1 antibody. CD90/Thy1 was detected in a paraffin-embedded section of rat brain tissue.

IHC analysis of CD90/Thy1 using anti-CD90/Thy1 antibody. CD90/Thy1 was detected in a paraffin-embedded section of mouse spleen tissue.