CD86 Rabbit Polyclonal Antibody

• Catalog Number: orb500820
• Category: Antibodies
• Description: CD86 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from the middle of rat CD86 (140-175/313aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 72-74 kDa
• Target: CD86
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD28LG2; FUN1; LAB72; Activation B7 2 antigen; Act
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: U937 (Human) Cell Lysate at 30 ug, Lane 3: Spleen (Rat) Lysate at 40 ug, Lane 4: Spleen (Mouse) Lysate at 40 ug, Lane 5: HL-60 (Human) Cell Lysate at 30 ug, Primary: Anti-CD86 (orb500820) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 72-74 kD, Observed band size: 72 kD.

Tissue/Cell: rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated (orb500820) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.