CD86 antibody

• Catalog Number: orb222751
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD86
• Clonality: Monoclonal
• Clone Number: BU63
• Tested applications: FA, FC, IHC-Fr, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: B-lymphoblastoid cell line ARH 77
• Concentration: 1 mg/ml
• Dilution range: Functional application: The antibody BU63 blocks mixed lymphocyte reaction (MLR) and binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD86 (B7-2). Flow cytometry: Recommended dilution: 5 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD86
• Entrez: 942
• UniProt ID: P42081
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-B7-2 antibody, anti-FUN-1 antibody, anti-LAB7
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD86 (BU63) purified antibody (low endotoxin, concentration in sample 3 μg/ml) GAM APC.

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD86 (BU63) purified antibody (low endotoxin, concentration in sample 3 μg/ml) GAM APC.