CD86 Antibody

• Catalog Number: orb1252437
• Category: Antibodies
• Description: CD86 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C86/1146
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1, kappa
• Immunogen: Recombinant human full-length protein was used as the immunogen for the CD86 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)Optimal dilution of the CD86 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD86
• UniProt ID: P42081
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: T-lymphocyte activation antigen CD86, Activation B
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)Optimal dilution of the CD86 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Daudi cell lysate using CD86 antibody (C86/1146).

Formalin-fixed, paraffin-embedded human melanoma stained with CD86 antibody (C86/1146).

Flow cytometry testing of human PBMC using CD86 antibody (C86/1146).