CD81 Antibody

• Catalog Number: orb1671615
• Category: Antibodies
• Description: CD81 Antibody
• Clonality: Recombinant
• Clone Number: 1.3.3.22
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizing mice with a B-Cell line derived from a Burkitt lymphoma.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CD81
• UniProt ID: P60033
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TSPAN-28, Tetraspanin-28, TSPAN28, CD81 antigen, 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD81 antibody 1.3.3.22. Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of 1.3.3.22 (orb1671615 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed Daudi cells with anti-CD81 antibody 1.3.3.22. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of 1.3.3.22 (orb1671615) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671615- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.