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Catalog Number | orb758795 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to CD80 |
Species/Host | Rat |
Clonality | Monoclonal |
Clone Number | RM80 |
Tested applications | Blocking, FA, FC, IHC, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells. |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | CD80 |
UniProt ID | Q00609 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | B7-1; Activation B7-1 antigen; B7; B7 protein; B7- Read more... |
Note | For research use only |
Application notes | This antibody has been used in multiple FACS experiments for various immunological applications, such as to highlight the robustness of the T cell priming process by activated Nlrc5-deficient dendritic cells (Rota et al, 2016), to demonstrate that allogeneic IgG combined with dendritic cell stimuli could induce anti-tumor T cell immunity (Carmi et al, 2015), to confirm the role of aquaporin-9-expressing neutrophils for the development of contact hypersensitivity (Moniaga et al, 2015), and to support the therapeutic potential of targeting B-cell Syk signaling in chronic graft-versus-host disease (Flynn et al, 2015). This antibody has also been used in Western Blot to study the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1-associated cytokines (Yue et al, 2015), as well as in in vitro blocking experiments to confirm the role of CD28 signals for IL-2-mediated survival and proliferation of both CD27(+) and CD27(-) γδ T cell subsets (Ribot et al, 2012). Furthermore, this antibody has been used, together with the anti-CD86 antibody, in numerous in vivo studies, such as in a NZB/W F1 mouse lupus model to clarify the role of CD80 and CD86 in murine Ab-mediated autoimmunity (Nakajima et al, 1995), in graft bearing recipient BALB/c mice to evaluate the roles of CD80 and CD86 costimulatory molecules in corneal allograft rejection (Kagaya et al, 2002), and in transgenic BALB/c mice to demonstrate the critical contribution of CD80 and CD86 in anterior chamber-associated immune deviation (Tsukahara et al, 2005). |
Expiration Date | 12 months from date of receipt. |
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